|DOI : http://dx.doi.org/10.14518/crals.2013.31.4.010|
1Department of Food Science & Biotechnology, Graduate school, Kyungpook National University, Daegu 702-701, Korea
2Food & Bio-Industry Research Institute, Daegu 702-701, Korea
*Corresponding Author: Sang-Han Lee, Tel. 82-53-950-7754, Fax. 82-53-950-6772, Email. firstname.lastname@example.org
|Received: December 12, 2013 / Revised: December 20, 2013 / Accepted: December 31, 2013|
|We studied antioxidant activity and inhibitory effect of α-glucosidase from aqueous, ethanolic and methanolic fractions of Galla rhois. In FRAP and ORAC assay for measuring antioxidant activity, we confirmed that Galla rhois extracts had strong antioxidant activity and ethanolic and methanolic extracts were relatively stronger than aqueous extract. We used trolox as a positive control. In order to measure the inhibitory effect of α-glucosidase, we compared acarbose and Galla rhois extracts. As a result of α-glucosidase inhibitory assay, aqueous, ethanolic and methanolic extracts of Galla rhois showed high inhibitory activitity and ethanolic and methanolic extracts were relatively stronger than aqueous extract. The 50% inhibitory concentrations (IC50s) of acarbose, aqueous, ethanolic and methanolic fractions were 0.45 mM, 0.53 μg/mL, 0.415 μg/mL and 0.37 μg/mL, respectively. These results suggest that Galla rhois extracts can be a clinically useful anti-diabetic ingredient, indicating that it needs to be fractionated and isolated and should be further investigated.|
|1.Finkel T, Holbrook NJ (2000) Oxidants, oxidative stress and the biology of ageing. Nature 408:239-247.
2.Heitzer T, Schlinzig T, Krohn K, Meinertz T, Munzel T (2001) Endothelial dysfunction, oxidative stress, and risk of cardiovascular events in patients with coronary artery disease. J Am Heart Assoc 104:2673-2678.
3.Valko M, Rhodes CJ, Moncola J, Izakovic M, Mazura M (2006) Free radicals, metals and antioxidants in oxidative stress-induced cancer. Chem Biol Interact 160:1-40.
4.Bae JS, Lee HS, Lee HY, Yoo BH, Kim TW, Kim YH, Kim TH (2012) Evaluation of acetylcholinesterase inhibitiory and antioxidative activities of Rhus javanica. Korean J Food Preserv 19:751-756.
5.Lee SH, Lee JK, Kim IH (2012a) Trends and perspectives in the development of antidiabetic drugs for type 2 diabetes mellitus. Korean J Microbiol Biotechnol 40180-185.
6.Lee SY, Kim IS, Park SL, Lim SI, Lim SI, Choi HS, Choi SY (2012b) Antidiabetic activity and enzymatic activity of commercial Doenjang certified for traditional foods. Korean J Biotechnol Bioeng 27:361-366.
7.Yoji H, Hiroshi N, Masahiro F, Shinji F, Yusuke S, Jiro N, Yutaka O (2012) α-Glucosidase inhibitor maglitol affects bile acid metabolism and ameliorates obesity and insulim resistance in diabetic mice. Metabolism 62:734-742.
8.Kim SJ, Yang HY, Lee SG (2009) Antimicrobial effect and cytotoxicity of Chinensis Galla. Korean J Orient Physiol Pathol 23:412-415
9.Cha BC, LEE SB, Rhim TJ, Lee KH (2000) Constituents of antioxidantive activity and free radical scavenging effect from Galla rhois (Rhus javanica Linne). Korean J Pharmacogn 31:185-189.
10.Choi I (2003) Antimicrobial activity of Rhus javanica extracts against animal husbandry disease-related bacteria. Korean J Soc Food Sci Nutr 32:1214-1220.
11.Benzie IF, Strain JJ (1996) The ferric reducing ability of plasma (FRAP) as a measure of "antioxidant power": FRAP assay. Anal Biochem 230:70-79.
12.Zulueta A, Esteve MJ, Frigola A (2009) ORAC and TEAC assays comparison to measure the antioxidant capacity of food products. Food Chem 114:310-316.
13.Yang YJ, Kim HJ, Kang SH, Kang SC (2011) Screening of natural herb resources for anti-oxidative effects in Korea. Korean J Plant Res 24:1-9.
14.Mosmann T (1983) Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assay. J Immunol Methods 65:55-63.
15.Klostergaard J (1985) A rapid extremely sensitive, quantitative micro-assay for cytotoxic cytokines. Lymphokine Res 4:309-317.
16.Kim SD (2013), α-Glucosidase inhibitor from Buthus martensi Karsch. Food Chem 136:297-300.